Figure 1(1): An engineered tissue construct of bioprinted cell-laden GelMA and sacrificial Pluronic F127.
Pluronic F127 is a sacrificial bioink that can be used for temporary support or to create channels, vessel or vasculature for bioprinting purposes. The recommended preparation provided in the user instruction below yields a streamlined printable biomaterial that supports 3D printed cellular constructs. However, preparation can be modified by users to suit their needs.
Storage & Handling
Pluronic F127 should be stored at 4°C until ready for use.
Instructions for Use
- Remove Pluronic F127 from storage. While still cold, load syringe with Pluronic F127.
- Allow Pluronic F127 to warm to room temperature.
- Print construct.
- To remove Pluronic F127 from printed construct, cool entire construct to 4°C over ice. Use a vacuum if necessary to remove material.
|Speed (mm/s)||Layer height (mm)||Nozzle Diam (mm)||Gauge|
|Crosslink (sec)||Print Temp (°C)|
*Note that the nozzle diameter print setting for this material is slightly larger than the diameter of the recommended gauge (150 μm).
Want to try a Practice Print?
Check out the information for Your First Bioprint for further instructions on preparing files for printing and general use instructions for Pluronic F127.
- For help adjusting print parameters please contact firstname.lastname@example.org.
- A fill volume change of more than 2 ml may affect pressure settings.
- A lower gauge size or tapered gauge will require lower pressure, while a higher gauge will require higher pressure for extrusion. Lowering the gauge size will also generally lower resolution