Pluronic F127

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Overview

Bio127 sacrificial structure

Figure 1(1):  An engineered tissue construct of bioprinted cell-laden GelMA and sacrificial Pluronic F127.

Pluronic F127 is a sacrificial bioink that can be used for temporary support or to create channels, vessel or vasculature for bioprinting purposes. The recommended preparation provided in the user instruction below yields a streamlined printable biomaterial that supports 3D printed cellular constructs. However, preparation can be modified by users to suit their needs.

Storage & Handling

Pluronic F127 should be stored at 4°C until ready for use.

Instructions for Use

  1.  Remove Pluronic F127 from storage. While still cold, load syringe with Pluronic F127.
  2.  Allow Pluronic F127 to warm to room temperature.
  3.  Print construct.
  4.  To remove Pluronic F127 from printed construct, cool entire construct to 4°C over ice. Use a vacuum if necessary to remove material.

Print Settings

Slic3r Settings
Speed (mm/s) Layer height (mm) Nozzle Diam (mm)   Gauge

4.0

0.2 0.2*  30

Print Settings

Pressure (psi)

 Crosslink (sec) Print Temp (°C)

40

0 25

*Note that the nozzle diameter print setting for this material is slightly larger than the diameter of the recommended gauge (150 μm).

Want to try a Practice Print?

Check out the information for Your First Bioprint for further instructions on preparing files for printing and general use instructions for Pluronic F127.

Notes

  1.  For help adjusting print parameters please contact info@biobots.io.
  2.  A fill volume change of more than 2 ml may affect pressure settings.
  3.  A lower gauge size or tapered gauge will require lower pressure, while a higher gauge will require higher pressure for extrusion. Lowering the gauge size will also generally lower resolution

Further Information

References

[1] D. B. Kolesky et al, “3D Bioprinting of Vascularized, Heterogeneous Cell-laden Tissue Constructs,” Adv. Mater., vol. 26, pp. 3124-3130, 2014.