BioBots Software Introduction: The Basics
Below is a guide for software parameters found on the BioBots interface. Each of these functions allows you to control your printer to develop complex, reproducible structures.
Use these controls to connect to your BioBot.
These controls allow you to upload various print files and to print them. Outputs such as timelapse, approximate total print time and print time help to keep track of a print.
Use these controls to calibrate your BioBot and to manually move the extruders and bed plate.
This settings for each extruder allows you to choose pressures for each loaded syringe. The BioBot 1 has pressure ranges from 1 psi to 120 psi. Pressure settings can have many effects on final prints, including material extrusion speed, resolution and even encapsulated cell viability. Generally, increased pressure leads to faster extrusion and lower resolution, while a decreased pressure causes slower extrusion and higher resolution.
Temperature controls on BioBots software regulates specified temperatures for an entire print. Extruder 1 has temperature ranges from room temperature to 120°C. Many thermoplastic support materials, such as polycaprolactone, require high temperatures provided by this extruder for printing. Extruder 2, designed mainly for cell-laden matrix reagents, has temperature ranges from room temperature to 80°C. This temperature range allows for matrix materials to be maintained at body temperature (37°C) during the printing process.
Use print crosslinking parameters to solidify photocurable materials as they print. These parameters control the 405 nm LED lights on the bottom of the second extruder. Below, the specific crosslink settings are described in more detail.
Print Crosslinking Parameters:
Frequency – The frequency setting offers users control over frequency of crosslinking. Set this to 1 layer for crosslinking between every printed layer. Set this to larger numbers for less frequent crosslinking.
Intensity – This intensity controls the intensity of the light when crosslinking between layers. The range of 0 – 100% correlates to 0 mW/cm² – 20 mW/cm² intensties.
Duration – The duration controls how long crosslinking between print layers will occur. Units are in milliseconds.
PostPrint Crosslinking Parameters:
Postprint crosslink parameters control crosslinking at the end of a print. Set desired intensity and duration to automate crosslinking after printing.
Manual Crosslinking Parameters:
Use this tool to manually toggle the light on and off with varying intensities. To keep the light on for an entire print, use this control.