FRESH Bioprinting Method
Figure 1: Kidney (left) and bifurcated tube (right) printed using a BioBot 1 and the FRESH method.
Freeform reversible embedding of suspended hydrogels, also known as the FRESH method, allows for the bioprinting of soft cell encapsulated materials such as collagen or alginate (1). The method utilizes a support hydrogel as a temporary, thermoreversible support that can be washed away after printing. The FRESH support reagent is comprised of processed gelatin microparticles with a Bingham plastic rheology. This biocompatible material offers temporary support of fragile materials used for bioprinting. This method is compatible with your BioBot 1 (check out the video!). Reagents for the FRESH method coming soon to www.biobots.com.
Figure 1: (adapted from (1)) Left: The FRESH Printing process involves printing a hydrogel such as collagen or alginate into FRESH support material, then washed the material away by heating the printed structure to 37 °C. Right: A model of a section of a human right coronary arterial tree from 3D MRI is processed at full scale into machine code for FRESH printing. An example of the arterial tree printed in alginate (black) and embedded in the gelatin slurry support bath
For collaborations or inquiries about the FRESH method, contact Adam Feinburg. For a protocol of the FRESH method with a BioBot 1, please contact email@example.com.
- Hinton, T. J. et al, “Three-dimensional printing of complex biological structures by freeform reversible embedding of suspended hydrogels,” Science Advances, vol. 1, no. 9, October 2015.