Gelatin Printing

Wikis > Gelatin Printing

Gelatin is a sacrificial bioink that can be used for temporary support or to create channels, vessel or vasculature for bioprinting purposes. Through a reversible thermal gelation, gelatin remains solid at room temperature and can be melted away at 37°C.  The recommended use for gelatin as a sacrificial material is as a FRESH support material. Below are results from printing gelatin individually.

Gelatin was dissolved at 60°C in formulations of 5% (w/v) or 10% (w/v), then loaded into a 10 ml BD syringe at volumes of 5 ml and allowed to cool to room temperature. Print files of lines (included below) were completed at varying speeds and pressures. Three prints were completed at each setting and analyzed with ImageJ software.

Print Settings
Gelatin Concentration (%) Pressre (psi) Speed (mm/s) Layer height (mm) Nozzle Diam (mm) Nozzle Shape Resolution (mm)

5

22 10 0.3 0.3 untapered 0.90 ± 0.10

5

22 12 0.3 0.3 untapered 0.59 ± 0.14

5

22 14 0.3 0.3 untapered 0.72 ± 0.10

10

63 14 0.3 0.3 tapered 0.43 ± 0.21
Untitled presentation (1)

Figure 1: Lines of 5% gelatin printed at 10 mm/s (A), 12 mm/s (B), 14 mm/s (C) and 10% gelatin printed at 14 mm/s (D). Scale bars at 0.5 mm.

After printed lines, z-stack tests were completed with both 5 and 10% gelatin at 14 mm/s. 5% gelatin was able to support itself up to 1 mm height, while 10% gelatin was able to support itself up to 5 mm.

G-Code Files

gelatin lines F600 Lines printed at 10 mm/s

gelatin lines F720 Lines printed at 12 mm/s

gelatin lines F840 Lines printed at 14 mm/s

pediatric bronchi 14mms  Pediatric Bronchi file used in z-height test