Figure 1: Live/dead image of human neonatal dermal fibroblasts encapsulated in bioprinted Gelatin Methacrylate
Gelatin Methacrylate is a gelatin-based hydrogel that reacts and crosslinks in the presence of LAP and BioBots blue light technology( 1,2). The recommended preparation provided in the user instruction below yields a streamlined matrix bioink that supports 3D printed cell-laden constructs. However, preparation can be modified by users to suit their needs.
Storage and Handling
GelMA should be stored at -20 °C. LAP should be stored away from light in a dry environment.
You Will Need
- Metal or UV-block needles (we suggest these)
- Petri dish or well plate for printing
- Sterile filters (we suggest these)
- Optional: Treated glass (See protocol)
Instructions for Use
- Measure 0.5% w/v LAP concentration and mix in cell media or PBS at 60°C. Mix until all LAP is dissolved.
- Measure 10% w/v GelMA concentration and add to solution from step 1. Mix at 60°C until all GelMA is dissolved.
- Heat solution from step 2 to 90°C and filter with a 0.2 µm syringe filter under sterile conditions.*
- Cool solution to 37°C, then mix in desired cell concentration in a sterile container. *
- Load cell-laden bioink into a 10 ml sterile luer lok syringe at 37°C. Cool on ice until bioink reaches room temperature, about 8 min.
Critical step: GelMA can take a while to cool to room temperature. It is important to cool the material on ice prior to loading to speed up this process.
- Load syringe into BioBots printer.
- Prepare your design file through Repetier Host, then upload the sliced gcode file to biobots software.
* For prints without cells, steps 3 and 4 may be skipped.
|Speed (mm/s)||Layer height (mm)||Nozzle Diam (mm)||Gauge|
*Critical step: Your needle can have significant effects on print settings. Be sure to use the suggested needle type or re-calibrate print parameters.
|Pressure (psi)||Print Temp (°C)|
- Issues printing? Check out our Troubleshooting Guide.
- Gelation time and gel stiffness can be adjusted by varying the concentration of GelMA or LAP. For help adjusting print parameters please contact firstname.lastname@example.org.
- A fill volume change of more than 2 ml may affect pressure settings.
- A lower gauge size or tapered gauge will require lower pressure, while a higher gauge will require higher pressure for extrusion. Lowering the gauge size will also generally lower resolution
for further questions or information about these products, please contact email@example.com
 D. B. Kolesky et al, “3D Bioprinting of Vascularized, Heterogeneous Cell-laden Tissue Constructs,” Adv. Mater., vol. 26, pp. 3124-3130, 2014.
B. D. Fairbanks et. al, “Photoinitiated Polymerization of PEG-diacrylate with lithium phenyl-2,4,6-trimethylbenzoylphosphinate: polymerization rate and cytocompatibility,” Biomaterials, vol. 30, no. 35, pp. 6702-6707, Dec 2009.