Multi-Extruder Bioprints

Wikis > Multi-Extruder Bioprints

Below are directions for a sample dual extrusion bioprint. This protocol is meant for educational purposes to help you learn how to use your BioBot 1 and familiarize yourself with the bioprinting process in general.

Load Your Syringe

  1.  Remove Pluronic F127 from storage. The material should be a liquid solution. While still cold, load your syringe with the material. For this first print, we suggest loading 2-3 ml.
    •  First place a syringe cap on your syringe.
    •  Then gently pour or pipette Pluronic F127 into the syringe
    •  Add the stopper, then invert the syringe and remove the syringe cap.
    • Carefully push the material to the top of the syring with a lab marker, then cap with a 30 gauge needle.
  2. Place your syringe into your BioBot. Allow Pluronic F127 to warm to room temperature before printing.
  3. Finally, place a petri dish into the bed plate.

Set Up Your Software

  1. Open Repetier Host and load your CAD File(Figures 1-5). We suggest this dual concentric file.
  2. For bioprints with Pluronic F127, you will want to set the slicer and print parameters to match the settings below.
Slic3r Settings
Speed (mm/s) Layer height (mm) Nozzle Diam (mm) Gauge
4.0 0.2 0.2* 30
Print Settings
Pressure (psi) Crosslink (sec) Print Temp (°C)
40 0 25

3.Slice your design and save the gcode file.

4. Upload your file to the BioBots software.

Print

  1. Connect your computer to your BioBot.
  2. Using software and manual controls, calibrate your BioBot to determine the zero positions.

Software Images

Software screenshot for multi-extruder bioprintFigure 1: Upload your .stl files into Repetier host. You should have one file for extruder 1 and one file for extruder 2. Rotate and orient properly.

 

Software screenshot for multi-extruder bioprintFigure 2: Place you files on the bed and center them.

 

Software screenshot for multi-extruder bioprintFigure 3: Label each file with the correct extruder for printing. Delta 1 refers to the left extruder on the BioBot 1. Delta 2 refers to the right extruder on the BioBot 1.

 

Software screenshot for multi-extruder bioprintFigure 4: Slice the file with desired slicing parameters. If a warning pops up that at least one object is outside the printable area, press no.

 

Software screenshot for multi-extruder bioprint
Figure 5: Your file is now ready to bioprint. Save the gcode file to upload into BioBots software.