Your First Bioprint

Wikis > Your First Bioprint

Congratulations! You are about to enter the exciting world of bioprinting! Your biobot offers you an accessible, easy to use platform for developing 3D tissues. To start, we suggest this first bioprint of a Pediatric Bronchi with our user-friendly Bio127 material. This first bioprint will allow you to familiarize yourself with your BioBot as well as the bioprinting process in general.

Bioprint with Bio127

Figure 1: Your Bioprint should look like this when completed(*blue food dye added for visual effects).

You will Need:

Instructions:

Load Your Syringe:

  1.  Remove Bio127 from storage. The material should be a liquid solution. While still cold, load your syringe with the material. For this first print, we suggest loading 2-3 ml.
    •  First place a syringe cap on your syringe.
    •  Then gently pour or pipette Bio127 into the syringe
    •  Add the stopper, then invert the syringe and remove the syringe cap.
    • Carefully push the material to the top of the syring with a lab marker, then cap with a 30 gauge needle.
  2. Place your syringe into your BioBot. Allow Bio127 to warm to room temperature before printing.
  3. Finally, place a petri dish into the bed plate.

Set Up Your Software:

  1. Open Repetier Host and load your CAD File. For your first print, we suggest starting with the Pediatric Bronchi file.
  2. For prints with Bio127, you will want to set the slicer setting to match the print settings below(Figures 2-4).
Slic3r Settings
Speed (mm/s) Layer height (mm) Nozzle Diam (mm) Gauge
4.0 0.2 0.2* 30
Print Settings
Pressure (psi) Crosslink (sec) Print Temp (°C)
40 0 25

3.Slice your design and save the gcode file.

4. Upload your file to the BioBots software.

Print:

  1. Connect your computer to your BioBot.
  2. Using software and manual controls, calibrate your BioBot to determine the zero positions.

Software Screenshots:

Bioprint Software screenshot layer height

 

 

 

 

 

 

Extruder1 Extruder2

 

Figure 2: Path height is set in print settings under layers and perimeters as the layer height for the print (left). Path length is set as the nozzle diameter for extruder 1 and extruder 2 under printer settings (right).

bioprint software screenshot speed

Figure 3: Print Speed is set in slic3r under print settings and speed. All speeds should be set at the same rate.
bioprint screenshot pressure adjustmentFigure 4: Print pressure and temperature are set in BioBots software after the sliced gcode file is saved and uploaded.

Troubleshooting:

The most common part of this process that users find trouble with is the calibration step. You may need to practice this a few times to get a feel for good starting heights.For further instruction or advice, please contact info@biobots.io.

What to try next:

 Once you’ve mastered printing with Bio127, try this same print with BioPCL!